Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACVAGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQK--YDFGPLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLA--AEEDFVQAAKELQD-MGAKNVMIKGHHAKNAQQETVNDYVLFES-G-KSFWLNAPFYPTDRINGTGDSLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE 3RM5 Chain:B ((19-292)) -NEKLPTVLSIAGTDPSGGAGIEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVFQTLESNLKDMKCNVIKTGMLTAA-AIEVLHEKLLQLGENRPKLVVDPVLVA--------KDIVSLITEKVAPFADILTPNIPECYKLLGEERKVNGLQDIFQIAKDLAKITKCSNILVKGGHI--------TDVL-FLGAEQKFIIFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVYGGIEYVQNAVAIGCDV----------------

General information: TITO was launched using: RESULT: Template: 3RM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1312 -45264 -34.50 -191.79 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -34.50 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_3RM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RM5-query.scw PDB file : Tito_Scwrl_3RM5.pdb: