Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEGQWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNGEDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKENPKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGIE-AREIEVAVLGNE-DVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQEYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIEELIQLGMNRYHQRQSFFEKNE 2ZDH Chain:C ((1-318)) MRVLLIAGGVSPEHEVSLLSAEGVLRHIP-FPTDLAVIAQDGR--WLLGEKALTA--LEAKAAP-------E----GEHPFPPPLSWERYDVVFPLLHGRFGEDGTVQGFLELLGKPYVGAGVAASALCMDKDLSKRVLAQAGVPVVPWVAVRKGEP----------PVVPFDPPFFVKPANTGSSVGISRVERFQDLEAALALAFRYDEKAVVEKALSPVRELEVGVLGNVFGE-ASPVGEVRYEAPFY---TKYTPGRAELLIPAPLDPGTQETVQELALKAYKVLGVRGMARVDFFLA-EGELYLNELNTIPGFTPTSMYPRLFEAGGVAYPELLRRLVELAL--------------

General information: TITO was launched using: RESULT: Template: 2ZDH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1773 -100782 -56.84 -321.99 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -56.84 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2ZDH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZDH-query.scw PDB file : Tito_Scwrl_2ZDH.pdb: