Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFVRNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLRNGDLVKVDMCIDLKGGISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLAIVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGWTAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY 2NQ7 Chain:A ((73-319)) -IKLLSSEDIEGMRLVCRLAREVLDVAAGMIKPGVTTEEIDHAVHLACIARNCYPSPLNYYNFPKSCCTSVNEVICHGIPDRRPLQEGDIVNVDITLYRNGYHGDLNETFFVGEVDDGARKLVQTTYECLMQAIDAVKPGVRYRELGNIIQKHAQANGFSVVRSYCGHGIHKLFHTAPNVPHYAKNKAVGVMKSGHVFTIEPMICEGGWQDETWPDGWTAVTRDGKRSAQFEHTLLVTDTGCEILTRR------

General information: TITO was launched using: RESULT: Template: 2NQ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1518 -152880 -100.71 -618.95 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -100.71 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2NQ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NQ7-query.scw PDB file : Tito_Scwrl_2NQ7.pdb: