Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRYSEKYRENGGIRVKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGAGKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTAKENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQL-----------SGGEQQRVSIARALAKNPKLLLCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRSVELNDHPSSIDEIVW 1MV5 Chain:A ((20-215)) ---------------------------------------DISFEAQPNSIIAFAGPSGGGKSTIFSLLERFYQPTAGEITIDGQPIDNISLENW----RSQIGFVSQDSAIMAG-TIRENLTYGLEGDYTDEDLWQVLDLAFARSFVENMPDQLNTEVGERGVKISGGQRQRLAIARAFLRNPKILMLDEATASLDSESESMVQKALD--SLMKGRTTLVIAHRLSTIVDADKIYFIEKGQI-----------------

General information: TITO was launched using: RESULT: Template: 1MV5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 774 -19512 -25.21 -105.47 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -25.21 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_1MV5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MV5-query.scw PDB file : Tito_Scwrl_1MV5.pdb: