Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSGSGKSTFLTISGGLQTPTSGEIVINGHSLTEL-PEKKRADLRFKEIGFILQSSNLIPFLTVKEQFELVDQVTSKKEDSTKIDSLLQSLD-IQSLKNSYP-------KDLSGGERQRVAIARALYNEPSLILADEPTASLDTDHAYEVVKLLVKEAHE--KQKATIMVTHDVRMIQWSDRVYRMEDGYLTEEEKKKE 2PMK Chain:A ((21-225)) -----------------------LDNINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDGHDLALADPNWLR-----RQVGVVLQD-NVLLNRSIIDNISLANPGMSV-EKVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNNPKILIFDEATSALD----YESEHVIMRNMHKICKGRTVIIIAHRLSTVKNADRIIVMEKGKIVEQGKHKE

General information: TITO was launched using: RESULT: Template: 2PMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 890 -96 -0.11 -0.49 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -0.11 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_2PMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PMK-query.scw PDB file : Tito_Scwrl_2PMK.pdb: