Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAILENFEEIKMTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVKDEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVVTANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVNGTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMKIAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLASVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGT--IGKDFNEY---SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARVVIITHKINKAQLENVSAELKKVS-EFDLLNTFKVLGE 3MTJ Chain:A ((5-440)) -------FQGMKPIHVGLLGLGTVGGGTLTVLRRNAEEITRRAGREIRVVRAAVRNLD-KAEALA--GGLPLTTNPFDVVDDPEIDIVVELIGGLEPARELVMQAIANGKHVVTANKHLVAKYGNEIFAAAQAKGVMVTFEAAVAGGIPIIKALREGLTANRIEWLAGIINGTSNFILSEMRDKGAAFDDVLKEAQRLGYAEADPTFDIEGIDAAHKLTILSAIAFGIPMQFERAYTEGISQLTREDVRYAEELGYRIKLLGIARRAENGIELRVHPTLIPERRLIANVDGAMNAVLVKGDAVGPTLYYGAGAGSEPTASAVVADLVDVTRLHTADPHHRVPHLAFQPDQLADTPILPMEAVRTAYYLRLRAF-RPGVLADITRILADSSISIDAMVQKE----QVDIILLTHVTLEKNVNAAIAKIEALDAVAGKVMRIRLED-

General information: TITO was launched using: RESULT: Template: 3MTJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2400 -260928 -108.72 -616.85 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -108.72 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_3MTJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MTJ-query.scw PDB file : Tito_Scwrl_3MTJ.pdb: