Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVLNPASVPLSLYIHMPWCVRKCPYCDFNSHAVPDGALSAELEQTYLKALVADFETQVELAQGRSIHSVFIGGGTPSLISAKGYAWLFEQLK-SLLNFEDDCEITLEANPGTLEHDPFAGYLEAGINRLSIGVQTFNTDHLQKLGRIHSANNAMDAIEQARQAGFKRVNVDLMHGLPEQTLEQALYDLKTAVEQGATHISWYQLTIEPNTVFFRTQP-VLPQD--EVLEDIQEQGEAYLKANGFINYEVSAWRKE-QP----------SAHNLNYWQ-F-GDYLAIGAGAHGKVTRPDGVYRFQKTRLPKDYLAKVPAEHLQ---MKKIEADELPFEFMMNALRLNDGVKAELYEQRTGLSLN-D---LNDVLTSLRSRKLLVEDSGRLACTEQGHIFLNSVLEEFL 1OLT Chain:A ((48-441)) -----YPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQH----KADQYLDALEQEIVHRAPLFAGRHVSQLHWGGGTPTYLNKAQISRLMKLLRENF-QFNADAEISIEVDPREIELDVLDHLRAEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQTPESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAFLTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGDC----YAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYSPIEQQWDLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFD

General information: TITO was launched using: RESULT: Template: 1OLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1841 -3076 -1.67 -8.31 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -1.67 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1OLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OLT-query.scw PDB file : Tito_Scwrl_1OLT.pdb: