Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFSRKQFALSAIFVAILATGGSFMLLHENADAKAAPTAAQQAATVDVAPVVSKTITDWQEYSGRLEAIDQVDIRPQVSGKLIAVHFKDGSLVKKGDLLFTIDPRPFEAELNRAKAQLASAEAQVTYTASNLSRIQRLIQSNAVSRQELDLAENDARSANANLQAARAAVQSARLNLEYTRITAPVSGRISRAEVTVGNVVSAGNGAQV--LTSLVSVSRLYASFDVDEQTYLKYIS----NQ---------RNSSQVPVYMGLANETGFTREGTINSIDNNLNTTSGTIRVRATFDNPNGVLLPGLYARIRLGGGQPRPAILISPTAVGVD-QDKRFVVVVDAKNQTAYREVKLGAQQDGLQIVNSGLQAGDRIVVNGLQRIRPGDPVTPHLVPMPNSQITANATPQPQPTDKTSTPAKG 2V4D Chain:G ((13-339)) -------------------------------------------PEVGIVTLEAQTVTLNTELPGRTNAFRIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLAS-------TQEQAQRYKLLVADQAVSKQQYADANAAYLQS-------KAAVEQARINLRYTKVLSPISGRIGRSAVTEGALVTNG---QANAMATVQQLDPIYVDVTQPSTALLRLRRELASGQLERAGDNAAK------VSLKLEDGSQYPLEGRLEFSEVSVDEGTGSVTIRAVFPNPNNELLPGMFVHAQLQEGVKQKAILAPQQGVTRDLKGQATALVVNAQNKVELRVIKADRVIGDKWLVTEGLNAGDKIITEGLQFVQPGVEVKTVP----------------------------

General information: TITO was launched using: RESULT: Template: 2V4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1349 -16598 -12.30 -53.37 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain G : 0.72 3D Compatibility (PKB) : -12.30 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_2V4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4D-query.scw PDB file : Tito_Scwrl_2V4D.pdb: