Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYTASCLCNGIQLRINAELEPIMVCHCKQCQKAQGAAFAAITQVQKSDLEIVQGENLLQAYFASPNKKRVFCKICGSPVWSERLDKPDVVRLRVGLINEEISTPVISHAFVNSQVKWYPICDTARQYPNGVENP 1X6M Chain:A ((35-130)) ------CSTNPVRVAVRAQTAHNHVCGCTKCWKPEGAIFSQVAVVGRDALEVLEGAEKLEIVNAEAPIQRHRCRDCGVHMYG-RIENRDHPFYGLDFVHTELS--------------------------------

General information: TITO was launched using: RESULT: Template: 1X6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 408 -44290 -108.55 -461.35 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -108.55 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_1X6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X6M-query.scw PDB file : Tito_Scwrl_1X6M.pdb: