Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPNQSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHSELAEQAMQLCANACQLCAAECSKHEHEHCQVCAKACLACAQACQAYRA 5FIG Chain:F ((4-108)) -------------YSEACIEACIDCMKACNHCFTKCLEE--QHHLSGCIRLDRECADICALAVKAMQTDSPFMKEICALCADICEACGTECGKHDHDHCQACAKACFTCAEQCRSMAA

General information: TITO was launched using: RESULT: Template: 5FIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 486 -79088 -162.73 -767.84 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain F : 0.79 3D Compatibility (PKB) : -162.73 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_5FIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIG-query.scw PDB file : Tito_Scwrl_5FIG.pdb: