Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNLNSPEQSDLSWLTIWSTFLNQAPFTAQTQAPEAACFLQQLIEASLQGDSCIDISPEQIETLGQ-L-----------------VI-SAEQ-AKSQVAPCVHDGQGLALYRYWNLEQRLAEQIRRLKQ-QPIQPVSCEEHLDL-----LTDPHQRTALQMVTRQSLSIITGGPGTGKTYTLARIIAVLSQA--IPHIRVAMAAPTGKAAQRMQEALQNSFNDPKLLESGLMSDELRNQSTQTIHRLLGMGHS-QTPRFNQKQPLPYDVIVVDEASMLDLNLATLLFEAVPESCRIILLGDANQLASVDVGAVLADLQQIEAL-----------------------------SENRVHLQTSRRFAEGALIGQVARFIQMQTSQIDHEQVLQQFEIDIVQVSELRSIPLEADMPDVVQLEYLPEESPIALETYYQKLMVGFQGYVQSLKAYLQDDHSLEHIENIVKSFDDYRILTAVRHGPFGLQQLNQYAERWLQQQLGIV--TLGGWYVGRPVMMTYNDYQLGISNGDIGLCFEHRTQPQQFEVYFP--SL-NKWVAANRLPKSIQSAFALTIHKSQGSEFTHTAVVLDQAAKNLLSQELIYTAITRAKKVVSLLVDPEALLQ-SFTVRTTRKSGLVEKVNRLVR 5LD2 Chain:D ((28-607)) ---------------------------VAGDEHPAVTLAAALLSHDAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMILCGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAVALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGKALRQLPLTDE---QKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDLPMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTGTHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKT-AVKTVFQQDF-------------------TDIEKRLLQSG--EDYIAMLEEALAGYGRYLDLLQA-------RAEPDLIIQAFNEYQLLCALREGPFGVAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQG---TRVWFAMPDGNIKSVQPSRLPE-HETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLSLYADERI-LSAAIATRTERRSGLAALFSS---

General information: TITO was launched using: RESULT: Template: 5LD2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2407 43776 18.19 84.84 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.72 3D Compatibility (PKB) : 18.19 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_5LD2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LD2-query.scw PDB file : Tito_Scwrl_5LD2.pdb: