Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISLEFLLASAIGLLVATGIYLILRARTFPVVLGLAVLGYAVNLFLFAMGRLQVSSPAILTETTNITDPLPQALILTAIVIGFATTAFIVQLALRSRYESGSDHVDAKEDISPTYDPREDEP 3RKO Chain:G ((1-100)) MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASALAFVVAGSYW-------------GQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG---------

General information: TITO was launched using: RESULT: Template: 3RKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 235 -44174 -187.97 -441.74 target 2D structure prediction score : 0.89 Monomeric hydrophicity matching model chain G : 0.72 3D Compatibility (PKB) : -187.97 2D Compatibility (Sec. Struct. Predict.) : 0.89 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_3RKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RKO-query.scw PDB file : Tito_Scwrl_3RKO.pdb: