Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSPRTQGYFFVLVTMCTWGGFTLFARLNAQWHISAWDIAALRFAIAFLILMPILIYKKD-LAFLWSKHAVILALIGGVIYCLTVYTAFLYAPAAHAAIFLNGCIPICTAIAAYILFRQPFDKHTWVSLAIMITALALMSALMLQS--STSAFGIGDLLLFISAIWWGIFTVLLKQW-KLSAWHSMASVAIWSAIIYLPIYLLFLPKHFMDADPLHLAVQSIFHGVFVVIIATLTYVAAIQRLGAFKTGSIVTLAPFIAAILAVPLLGESLSPSIVVGLVGMGIGALQPWRWFRQDSLTQKIKQQNTRN 5I20 Chain:D ((2-285)) --SRSSATLIGFTAILLWSTLALATS-STG-AVPPFLLTALTFTIGGAVGIAAGLARGVG-LRQPW-PVWVHGIGGLFGYHFFYFSALKLAPPAEAGLVA-YLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGA-AGGFGFAPEYVPGYLAAAACAVIWSVYSVASRRFARVPTEVVAGFCLATAALSALCHILFEPS---VWPVGSEWL-AVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGALAIACALIVGGAAVATLLA----------------

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1358 -258870 -190.63 -937.93 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.72 3D Compatibility (PKB) : -190.63 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: