Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQLELKKVIRPVILIVALMIAVYTIVHLWNYYNAAPWTRDGRVRG-DVIQVSSDVAGLVTEVLVQDNQTVKKGQVLFKIDVSRRALDVEQAKSDLAKAKAAYAQAQ-------------------------AGLAQAKANLIKSSTNIKLAEKNANRYSNLMDGAISKQ-EQDQVFATRDQSHAEHEQLQA--------------AI----------QQAEATIKQQQALVEAATSNLHLAELNMHRAAVVAPADGTLSNFDLRPGNYVQVGQAVAALLDRKQLYVVGYFEETKLDRIHIGDEATVQLMGDR-QKIKGHVQGIASGIEDRERSSSSKLLANVNPTFSWVRLAQRVPVKIVLDEAPKNELAFVSGRTAKVHIIEK 4TKO Chain:B ((7-348)) -----------------------------------YAITNAVFVKADELSYLSFRVSGKVIEVYKDLGDYVKRGEALAKLDPTYYELEKRTLEKKMSALLEKKKALEIKIQKLEKGLHISLSAKKLKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQKGLIPRRKFEEVDTNLKVLLHEREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKKELSSLEEEIKSLKERIKTAEQKIKDTVLIAPFDGVVAKRFISRGDVVRAGQPAFALVNPESFYVEVLLEETKLKGVKVGNKAYVRLDAYPDILFEGVVEEISP--------------------------VQRIPVKIKITKGDL--SLLRVGMGGEVEIRR-

General information: TITO was launched using: RESULT: Template: 4TKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 722 45646 63.22 169.69 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 63.22 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_4TKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TKO-query.scw PDB file : Tito_Scwrl_4TKO.pdb: