Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAISSFGKILTVLDLFSVSRTVINVDIICEELGLSKPTSYRYLKELVSADLLKRINGTSGDYTLGSKIAVLDYVSRTTDPLVQISTPFMRNIVERTELCCLLTYLNDDYCIDIHHEIFKDTELLSYGRGCPRPIYVGASPKTMVSHLSKQRMHDYYERFQHELSESGFAVDEPSFIQRMRKIKKQGFYFSQGEIDPNVSGLAVPVRFSSKEVPLALTLVASKNRFDFLNIEKLIEILQENAALIEQRFMELSEKGEI 5H1A Chain:B ((28-268)) --DSMLARVVRVLETFNVDRTAQTASDIGRRAALPSSTAHRVVDEMVLVGILERGI--DGKVRLGMRLWELALRGSMALRLRQVALPHMERVQQRVREHTQLAVLEHNEVLFLERLSHHEAVSNLARVAGRLPVHASSSGLMLLAHAGPEVREEVLSKPLP-RVGPGTVTDPEALRRLLANAYRAGYVAAPGYIEAVATGIAVPIRS-EGVVIAALSAVQPLQNAV----EPTVEILREAAVGIETDLRAS------

General information: TITO was launched using: RESULT: Template: 5H1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1094 -162577 -148.61 -674.59 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -148.61 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_5H1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H1A-query.scw PDB file : Tito_Scwrl_5H1A.pdb: