Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNQLPVINLMGPTASGKTALACELYERGNFELISVDSALVYKDMDIGTAKPTREEQELYPHHLIDIITPLEVYSAAQFVEDACALIDEMHSRGKTPILVGGTMLYFKALLEGLSSNLPSADANVRAAIEEKAANEGWQAVYDELVAVDPAAGVKFKVSDKQRIIRALEVYHITGQPITKLQAEQPKNVPYRYTFHNYALLPDRVE-LHQRIEQRLSKMWDIGFLSEVESLIEKYDLDENLPSMRSVGYRQALEFLLKSDMSLKKKQEMEDKALFATRQLAKRQYTWLRSLQEIHDFKTYLTIKQAKEDLRNSYG 2ZM5 Chain:A ((12-293)) ------AIFLMGPTASGKTALAIELRKILPVELISVDSALIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIPLLVGGTMLYFKALLEGLSP-LPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARIHPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQ--VHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSY-LEGEISYD---EMVYRGVCATRQLAKRQITWLRGWEGVH--------------------

General information: TITO was launched using: RESULT: Template: 2ZM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1284 -133141 -103.69 -473.81 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -103.69 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_2ZM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZM5-query.scw PDB file : Tito_Scwrl_2ZM5.pdb: