Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTKKDLLQQRMLIDQLRTQNSLSPQNAAKLGQSIASSWERSASA------AIPKERFAAPLLEKKSASQNALDLALSQCADDLRHIAEQSSMVIAVGDIGSTIIWTASSAQMQSAAERVHFVQGGQWREEFVGTNALALSLKTQQSSCVFSNEHYMESIHDWVCYAAPIIDPYSKQTLGVVDLSTTWKNHNTLGILAAERCASIIQSALLEQQRQQLHIRAFSTPQVKFNGKS-LLLTPRQIEILTILALCPHGLTLENLYQALYGERKVSMGTLKAEMSQLRDILGGLLGSRPYRLLVHVEADFLQAEQALDAGYVASALQLYAGVFLAKTESPFLCAWRDCLESRLSDAIFKAQETDLLLKHLAHFPEAIDAVERLMELLPSEHPAHQLLVKYIDSPKLS 4LRZ Chain:E ((13-306)) -------------------------------SPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYM-APRECALFILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQVLWNWAFCATPLFDSKGRLT-GTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFIN--------AQAARVLRLDATASQGRAITELLTLPAVLQQAIKQA--HPLKHVEATFESQHQ---------------------------------------------------------------FIDAVITLKPIIETQGTSFILLLHPV---

General information: TITO was launched using: RESULT: Template: 4LRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1311 2470 1.88 8.60 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain E : 0.63 3D Compatibility (PKB) : 1.88 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4LRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LRZ-query.scw PDB file : Tito_Scwrl_4LRZ.pdb: