Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTSKFNQFVRDIYQTNNFIPLHEPRFLGNEKKYVLDTIDSTFVSSVGAYVNDFESKIQHFTGCAKAIATVNGTAALHIALLLAGVKRDDIVITQALTFVATCNALSYIGAEPLFIDVSLKTMGLCPKALDTYLQENAFLDDQGVCKHTATNKRISAIVPMHTFGHPVEIDELQEVVTRWNIALVEDAAESLGSYYKGKHTGIFGLVSALSFNGNKVITTGGGGMVLCQDEELGVRAKHITTTAKIPHPYEFYHDENGFNYRLPNLNAALGCAQMESLEGFLSKKRDLAHQYQNFFKDT-DISFFVEPENCQSNYWLNAIICKN---------------------KTQRDLILDETNSNKVMTRPIWTLMTRLPMYENALQGDLTNSLWLEERVVNIPSSVPLE 4ZTC Chain:A ((1-368)) -----------------MRFFLSPPHMGGNELKYIEEVFKSNYIAPLGEFVNRFEQSVKDYSKSENALALNSATAALHLALRVAGVKQDDIVLASSFTFIASVAPICYLKAKPVFIDCDE-TYNIDVDLLKLAIKEC--------------EKKPKALILTHLYGNAAKMDEIVEICKENDIVLIEDAAEALGSFYKNKALGTFGEFGVYSYNGNAIITTSGGGMLIGKNKEKIEKARFYSTQARENC-LHYEHLDYGYNYRLSNVLGAIGVAQMEVLEQRVLKKREIYEWYKEFLGEYFSFLDELEN-S-RSNRWLSTALINFDKNELNSCQKDINISQKNITLHPKISKLIEDLKNKQIETRPLWKTMHAQEVFKGAKAYLNGNSELFFQKGICLPSGTAM-

General information: TITO was launched using: RESULT: Template: 4ZTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1999 -175276 -87.68 -506.58 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -87.68 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4ZTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZTC-query.scw PDB file : Tito_Scwrl_4ZTC.pdb: