Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCCFVVYGGVLNMSDKKTSLVICFTPLQMLIAEKIVAQQP--SVDLIVIALNNNDKYKYYYNIFQH---PNIAKQYYLFDNNKSRFFKFINIIK-F-NENFKLEKFYENIYISSIDNKYTHNILSKVNFNNLYTYDDGTANIVKNSIYFKQTFE---SKLKDIVLKLIGIRFNLNKIKKLSRKHYTIYKGIQNIVEQT----EYVSIIEKKEEDRNIFIDQEIKIFLGQPLKDIDKNFDTLALKKLLVMEEVNYHFKHPRETDEA-FFEEIKTSYIFEDFFAKELSKY--RKVIIYTLSSTAALNIISLDNVEIRLIKNNTVEIKYP--DLVQLFIKSGATVIDMDSIK 2YK4 Chain:A ((2-322)) ----------------PVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEWAYF-FHLPYG--LNKSFNFIPTMAELKVKA-MLLPKVKRIYLASLEKVSIAAFLSTYPDAEIKTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIG-DWSIAKTRNASDEHYTIFKGLKNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKE-----ISEKAAKNFNIQYVAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNVHVYALKPASLPEDYWLKPVYALFTQSGIPILTFDD--

General information: TITO was launched using: RESULT: Template: 2YK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1176 -11804 -10.04 -39.09 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -10.04 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_2YK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YK4-query.scw PDB file : Tito_Scwrl_2YK4.pdb: