Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTESTTPKFAIPELPMQIQTIRQYLPHRYPFLLVDRVTEVTDN-SIIGYKNVSINEEFLQGHFPEYPIMPGVLIVEALAQVSGVLGFIMNNETPKPGSLFLFAGAERVRFKKQVVAGDQLVLKSELVMQKRGIYKYNCTASVDGIVAATAEIMISHQKTEQA 5BUW Chain:B ((20-163)) ------------THTLHIEEILDLLPHRFPFLLVDRVLDFEEGKFLRAVKNVSFNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSRGK-LEPGELYYFAGIDEARFKRPVVPGDQMIMEVEFVKERRGLTRFTGVAKVDGEIVCTATMMCARS-----

General information: TITO was launched using: RESULT: Template: 5BUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 684 -44740 -65.41 -312.86 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -65.41 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.629

(partial model without unconserved sides chains): PDB file : Tito_5BUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BUW-query.scw PDB file : Tito_Scwrl_5BUW.pdb: