Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMNTQREQLIHTWLTSVLENDQFQIAYLAGDASFRRYACIQLQNKTYMLMDAPPE--KEDCVPFVTIDEFFAGHGVRVPQIVAKDLNQGFLLLEDFGDVLLSTLLNDET--VDQYYEQSFKQLIHLQSINGAEHFLAYSYEKLLSEMQLLTDWMLPS-LDIHPTAEQKKTIDDAFDFLAQAALAQPQVIVHRDFHSRNLMKIAN---EEELGVIDFQDAVIGADTYDLISITRDAYVQWNAERVYQWFKVFYELLPASARQNRSFDQFKRDADLMAIQRHIKILGIFVRLFERDGKSGYLKDLPRVMWYLLEESRGYHELDDFMAFIHSSVMPKFIEKYGPYEVAA 3CSV Chain:A ((4-326)) -----SREDEIRDFLAT-HGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTP-ELARLDPETLSEMTRLAFSEYRYAILGDAA-EDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATG------VDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLA-HPALASAAEEILNALPAPAPE---------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1450 -43229 -29.81 -140.81 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -29.81 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CSV-query.scw PDB file : Tito_Scwrl_3CSV.pdb: