Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNLKSSHSTAFTPSSPAERYAEALASGQFMADEAQAQAVQELDRVWKELLNRYKASKKAFRRFRRQTSPKGVYMWGSVGRGKTWLMDQFYESVPFRRK--TRMHFHHFMQH-VHKELNKLSGQRNPLDIVADQI-YKDAVVICFD--EFFVSNVTDAMILSDLFQKLFVRGVTLIATSNIAPDGLYKNGIHRDRFIPTIEMVKKNCVVLNVDAGVDYRLRVLKQ-AQLFKSPLGNEAQSWISECYTALTHTQTNSHEPIVINNRVVETLGHTEDVLWCEFSELCLKPRSPADFIEIA-NIYNTVLVSNVPHLTDFLSEGTRRFIYLVDEFY-DRGVKLLLTSQDSIIDIYQGEKLAFEIERTRSRLLEMQSDEYLHSEHRHIDATKTS-- 1L8Q Chain:A ((2-324)) -DFLNPKYT--LENFIVGEGNRLAYEVVKEALENL-----------------------------GSLYNP--IFIYGSVGTGKTHLLQAAGNEAKKRGYRVIYSSADDFAQAMVEHLKKG--------TINEFRNMYKSVDLLLLDDVQFLSGKERTQIEFFHIFNTLYLLEKQIILASDRHPQKL--DGVS-DRLVSRFE----GGILVEIELDNKTRFKIIKEKLKEFNLELRKEVIDYLLEN-----------------TKNVREIEGKIKLIKLKGFEGL---ERKERKERDKLMQIVEFVANYYAVKVEDILSDKRNKRTSEARKIAMYLCRKVCSASLIEIARAFKRKDHT-TVIHAIRSVEEEKKRKFKHLVGFLEKQAFDKIC

General information: TITO was launched using: RESULT: Template: 1L8Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1347 13428 9.97 43.31 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.97 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_1L8Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L8Q-query.scw PDB file : Tito_Scwrl_1L8Q.pdb: