Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFTSRLKKELFIKAQNLVPQHQLSRVVGKVAASENPILKAAVIHAFKTKYGIDLSIAEQGNALKYKSFNDFFTRALKDGVRLVDENPDSIVSPADGAISQIGKITAGKVFQAKGQSFSVEKLIGDPQLAQPFQEGEFATVYLSPRDYHRVHMPFSGILTETLYVPGELFSVNQVTAENVPGLFARNERMVCLFDTELGRMAVVLVGAMIVAGIETVATGKVKPSGRIELQHHELKLEKGAELGRFYLGSTAIILFEKDKIEWEKRFKAESVVVMGERMGHTL 2F3G Chain:A ((19-125)) ---------------------------------------------------------------------------------------TIEIIAPLSGEIVNIEDVPDV-VF-------------AE----KIVGDG----IAIKPTG-NKMVAPVDGTIGKIF-----------------------ETNHAFSIESDSGVELFVHFGIDTV---ELKGEGF-K----RIAE-EGQRVKVGDTVIEFDLP----------------------------------

General information: TITO was launched using: RESULT: Template: 2F3G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 465 -51642 -111.06 -482.63 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -111.06 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.376

(partial model without unconserved sides chains): PDB file : Tito_2F3G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F3G-query.scw PDB file : Tito_Scwrl_2F3G.pdb: