Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMSPIIHSLLDTDLYKFTMLQVVLHKFPQTHSVYHFRCRNLEDTVYPLVDILDDLNEQLDHLCNLKYKEDELQYLRKLRFIKSDFVDYLELFQLKRRFIHASIDEEGRLDIRIEGPMVQAMMFEIFVLAIVNELYFSRIKTDEVWAEGERRLQAKLELIQQYEKAQQPNDPPFLVSDFGTRRRYSFEWQKHVVAAFHNTVPNVFRGTSNVLLAKELNITPIGTMAHEFLQAFQALDVRLRDFQKAALEMWVQEYRGDLGIALTDVVGMDAFLRDFDLYFAKLFDGLRHDSGDPYEWGDKAYAHYRKLKIDTKTKMLTFSDGLNLPKAWELHQYFKDRFQVSFGIGTNLTNDMG-QTPLNIVLKLVECNGQSVAKISDSPGKTMTDNDTFLAYLRQVFQIEELDEAI 3OS4 Chain:A ((5-395)) --ASPILTSLLDTDAYKLHMQQAVFHHYRHITVAAEFRCRSDE----LLGVYADEIRHQVTLMGQLALTSDEFIYLSSLPFFQDDYLHWLRDFRFKPEQVSVA-VHDGKLDIRIAGLWCEVIMWEVPLLAVISEIVHRRRSTQVTTDQAVQQLRTKLEQFNALSADI--DITHFKLMDFGTRRRFSREIQHTVVSTLKDEF-PYLVGTSNYDLARTLALAPVGTQAHEWFQAHQQISPTLANSQRVALQVWLDEYPNQLGIALTDCITMDAFLRDFDLAFANRYQGLRHDSGDPIEWGEKAIAHYEKLGIDPMKKVLVFSDNLDLEKALFLYRHFYQRIKLVFGIGTRLTCDIPDVKPLNIVIKLVECNDKPVAKLSDSPGKTICQDPAFVDQLRKAFALP------

General information: TITO was launched using: RESULT: Template: 3OS4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2162 -226298 -104.67 -580.25 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -104.67 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_3OS4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OS4-query.scw PDB file : Tito_Scwrl_3OS4.pdb: