Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIFEAL-RESHENQRNLSEQLIQTHG-----------LTEERKELFDALKNELYAHSVAEDRYLYIPLMFDDV--GLDITRHALSEHHEMDELVGQLEKTDM----------SSPSWLATAKQLSEKVHHHLKEEEHKFFQQAGKILKDSEKETLGNKYLKEYKKYKKQQ 5FNP Chain:A ((74-218)) -EIIDHIIVRYHDRHREQLPELILQATKVERVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEHDEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA-------------------------

General information: TITO was launched using: RESULT: Template: 5FNP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 355 12707 35.79 105.01 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 35.79 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.653

(partial model without unconserved sides chains): PDB file : Tito_5FNP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FNP-query.scw PDB file : Tito_Scwrl_5FNP.pdb: