Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIEFIATSKLPTAFGEFNISVFQDPVTGEEHVALSKGLENPPTDPVLVRVHSECLTGDAFASLKCDCGPQLQATQKLINEAGQGVILYLRQEGRGIGLTNKIRAYALQDQGHDTVDANLLLNLPADARRYDMCSIMLDHLKVKEVKLITNNPLKIQALKDQGINVVDRVPLTVGRNPFNEQYLKTKRERMAHLYQKDDF 2BZ1 Chain:A ((1-173)) MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISG-HTPVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIV--------------------------

General information: TITO was launched using: RESULT: Template: 2BZ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 817 -89096 -109.05 -515.01 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -109.05 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_2BZ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BZ1-query.scw PDB file : Tito_Scwrl_2BZ1.pdb: