Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTITVAAAVILNEQNELLLVRKRNTQAFMQVGGKLEPNEAPETAIQREILEEIGSPCVIEQFIGRFETAAANEPDHKLISHLYLVRLKQSPQIAAEIAEMKWVKFNDS-ETKLAPLTKEIVIPWCEQNLSITL 1VC9 Chain:B ((3-121)) ----LGAGGVVFNAKREVLLLRDR-MGFWVFPKGHPEPGESLEEAAVREVWEQTGVRAEVLLPLYPTRYVNPK--GVEREVHWFLMRGEGAPRLEEGMTGAGWFSPEEARALLAFPEDLGLLEVAL--------

General information: TITO was launched using: RESULT: Template: 1VC9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 585 -68028 -116.29 -576.50 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -116.29 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_1VC9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VC9-query.scw PDB file : Tito_Scwrl_1VC9.pdb: