Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLNSSIDKKMTTLNILFKTTSLCLAMALLVGCNDNDDNDSSTPAPRSNQTVNILAFNDFHGNLEPPKRYVEAANPNDASQTVRIPVGGVSYFADAIKKLKAQNPNNVVVSAGDLISASPLTSSLFLDEPTIEVMNDIQIDFNAVGNHEFDRGTDELRRLQNGGCQQYTSTAPCQINKNFSGAKFNFLAANVAMKNDTS-KTIFPAYKVKTYGGIPVAFIGLTLKATPSIVSAAGIKDVEFRDEADTVNALIPELQKQGIEAIVVVVHEGAAPSTKLNQKTCDGLSGPILGILDRLNPAVDIVVSGHTHQSYICDY-------------ATKNPAKPFLLTSAGQYGTLITDIKVGLDGKTGDIIKKDAKQIPVQSEAYTSGTTTVSLTDLYQKFSKTPSIEAILDKYRQAVTTISGRVVGTSTAVVSR--TQVESGESPLGDMIADAQQAAALQASNQGSDFTLMNPGGVRADLLINSSNQITFGDIFAVQPFGNSIVTLSLTGKQIRDVLEQQWSGANANSPRILQPSKELSYQYAANTSVSPRASNIMVA----GSPLVDTKVYRVTVNSFLADGGDNFTVLKEGQNR--VGGGQDIDALESYVAQNSKDSPLIIPATTRIKVIK 2Z1A Chain:A ((28-531)) -------------------------------------------------FTLTLVHTNDTHAHLEPVELTL---------SGEKTPVGGVARRVALFDRVWARAKNPLFLDAGDVFQGTLYF-NQYRGLADRYFMHRLRYRAMALGNHEFDLGPGPLADFLKG-------------------ARFKVVSANVDASREPRLKGLFAPYAVVVVGGERVGIIGLTTPDTREISNPG--PTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDL---------------KLARRL-VGVQVIVGGHSHTLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAKG-ELLAYKGEALLMTP----------------EAAPEDFFAKEALLAYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLITDGMLWKTRN---AGTQIALQNGGGIRASIP---KGPITVGKVYEVLPFGNTLVVMDLKGKEILAALENGVSQWENTAGRFLQVS-GLRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNNFIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKELK---VVEAGLEGRIEVL-

General information: TITO was launched using: RESULT: Template: 2Z1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3022 -42249 -13.98 -87.65 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -13.98 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2Z1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1A-query.scw PDB file : Tito_Scwrl_2Z1A.pdb: