Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIYILGSAAGGGCPQWNCNCPNCHGVRTGTINAKVRTQSSIAISENGVDWILLNASPDIRQQLFDFKAAQPARKLRDTGITNVILMDSQLDHTTGLLTLREGCPMNVWCTEMVYQDLTTGFPVFNMLKHWNGGLQYHQVDPKQAFIIDGFENLEFLPLIIQSAAPPYSPHRHDPHEGDNIALIIKDHKTQKQLFYAPGLGKIDDQIMQIMQDSDCVMIDGTLWTDDEMQQTGVGKKTGREMGHLYISGEGGSLSYLNQLSTPKKVLIHINNTNPILNEDSAQFAELKANGVEVAFDGMQIEL 4Z5Y Chain:A ((2-304)) MYIQVLGSAAGGGFPQWNCNCVNCKGYRDGTLKATARTQSSIALSDDGVHWILCNASPDIRAQLQAFAPMQPARALRDTGINAIVLLDSQIDHTTGLLSLREGCPHQVWCTDMVHQDLTTGFPLFNMLSHWNGGLQWNRIELEGSFVIDACPNLKFTPFPLRSAAPPYSPHRFDPHPGDNLGLMVEDTRTGGKLFYAPGLGQVDEKLLAMMHGADCLLVDGTLWEDDEMQRRGVGTRTGREMGHLAQNGPGGTLEVLDGFPRQRKVLIHINNTNPILDENSPERAEVLRRGVEVAFDGMSIEL

General information: TITO was launched using: RESULT: Template: 4Z5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1815 -170581 -93.98 -562.97 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -93.98 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_4Z5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z5Y-query.scw PDB file : Tito_Scwrl_4Z5Y.pdb: