Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYRTIETILVDIPTIRPHQLSVTTMRTQTLVLVKITTTDGIVGWGEATTIGGLNYGEESPESVKANIDTYFAPLLTSVKDLNVAQTLKLIRKNINGNRFAKCAIQTALLDIQAKRLGVPLSEVLGGRLRNSLPVLWTLASGDTEKDIAEARKMIELKRHNTFKLKIGARPLQQDVDHVIAIKKALGADVSVRVDVNRAWSELECIHGIQQLQDGGIDLIEQPCAIQNTEALARLTHRFDVAIMADEALTGPDSAYRIAKNHGADVFAVKIEQSGGLIEACEVGKIAGLAGIDLYGGTMLEGPVGSIASAHVFTTFETLAFGTELFGPLLLTEEILKGPLRYENFELHLPTAPGLGIEIDEDKIEKLRR 3CT2 Chain:A ((10-374)) ---SIETIIVDLPTI-----------NQTLVLIRLRCADGIEGLGESTTIGGLAYGNESPDSIKTNIDRFVAPLLIGQDASNINAAMLRLEQSIRGNTFAKSGIESALLDAQGKRLGLPVSELLGGRVRDALPVAWTLASGDTAKDIAEAQKMLDLRRHRIFKLKIGAGEVDRDLAHVIAIKKALGDSASVRVDVNQAWDEAVALRACRILGGNGIDLIEQPISRNNRAGMVRLNASSPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRATLRTAAIAEAAGIGLYGGTMLEGGIGTLASAHAFLTLNKLSWDTELFGPLLLTEDILAEPPVYRDFHLHVSKAPGLGLSLDEERLAFFRR

General information: TITO was launched using: RESULT: Template: 3CT2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2154 -201193 -93.40 -568.34 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -93.40 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_3CT2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CT2-query.scw PDB file : Tito_Scwrl_3CT2.pdb: