Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSVHQLQGVGSASAALLEKLNIFTTDDLLFHLPRDYEDRSTIIPMNQLMVGRTYLLEGEVKSVDFPPGKRKS-MAALIQDEFGKVTLRFYH-IYKNLTDKIKPGNRLRIFGEVRVGARGLELYHPEIQLINEHTTLPKT-----QLTAIYPSTDGLTQAKLREYVKQALKHHSDALPELLPKQYTNGYAL---KEALHYIHEPPIDANMIQLAQDSHPAQQRLIFEELVAHQISL-LTRRAYIRQIASPAFPSSKVLAKKLLEALPFQMTNAQKRVSKEILNDLKQHQPMLRLVQGDVGAGKTLVAAVAACHALEADWQVALMAPTEILAEQHYLNFKRWFEPLGITVAWLSGKQKGKARAHAERQIKEGHAELIVGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLALRNKGAEQLTPHQLVMTATPIPRTLAMSAYGDLDTSIIDELPPGRTPIQTVTIPLDRREEVLQRIASNCREGKQAYWVCTLVEQSETLDAQAAEATYQEM-KERFPELNIGLVHGKMKADEKQAVMQAFKNNELQLLIATTVIEVGVDVPNASIMVIENAERLGLSQLHQLRGRVGRGAKASFCVLLYKPPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMGFRVARLERDDHLLSQAHYVAQQVLKDYPEQADALLKRWLPEAPRYAYV 1GM5 Chain:A ((115-755)) -TDIQYAKGVGPNRKKKLKKLGIETLRDLLEFFPRDYEDRRKIFKLNDLLPGEKVTTQGKIVSVETKKFQNMNILTAVLSDGLVHVPLKWFNQDYLQTYLKQLTGKEVFVTGTVKSNA-----YTGQYEIHNAEVT-PKEGEYVRRILPIYRLTSGISQKQMRKIFEENIPSLCCSLKETLPERILEKRKLLGVKDAYYGMHFPKTFYHL-------EKARERLAYEELFVLQLAFQKIRKEREKHGGIPKKIEGK-LAEEFIKSLPFKLTNAQKRAHQEIRNDMISEKPMNRLLQGDVGSGKTVVAQLAILDNYEAGFQTAFMVPTSILAIQHYRRTVESFSKFNIHVALLIGATTPSEKEKIKSGLRNGQIDVVIGTHALIQEDVHFKNLGLVIIDEQHRF-----EALMNKGKMVDT---LVMSATPIPRSMALAFYGDLDVTVIDEMPPGRKEVQTMLVPMDRVNEVYEFVRQEVMRGGQAFIVYPLI--------KSAVEMYEYLSKEVF---KLGLMHGRLSQEEKDRVMLEFAEGRYDILVSTTVIEVGIDVPRANVMVIENPERFGLAQLHQLRGRVGRGGQEAYCFLVV-GDVGEEAMERLRFFTLNTDGFKIAEYDLKTRGPGE----KQHGLSGFKVADLYRDLKLLEW---------------------------------

General information: TITO was launched using: RESULT: Template: 1GM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2940 39177 13.33 64.33 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 13.33 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1GM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GM5-query.scw PDB file : Tito_Scwrl_1GM5.pdb: