Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFLQRSNQHSNNNRLKRMKSHLRVHYFQHIAGEGFGSCYSFLKAHHATITATEFFALPVDLPLEIEALP-YIEDVDLLLIMGGTMSVNDE-ANYPWL--KIEKRWIRRYLAAGKPAIGLCLGGQLIANALGAAVSRNRYQELGWSTVQRVPNLPKESFL--LPEKINVMQWHSETFEIPKGAIHLAENSVCRNQMYQIGSNVLGFQFHPEMTPKVLNLLLENEQELSIFK--GEYVQSLDELHHCDIQKFEQGNQLLNRAIEFVVNQ 3L7N Chain:A ((1-234)) -----------------------MRIHFILHETFEAPGAYLAWAALRG--HDVSMT--KVY---RYE-KLPKDIDDFDMLILMGGPQSPSSTKKEFPYYDAQAEVKLIQKAAKSEKIIVGVCLGAQLMGVAYGADYLHSPKKEIGNYLISLTEAGKMDSYLSDFSDDLLVGHWHGDMPGLPDKAQVLAISQGCPRQIIKFGPKQYAFQCHLEFTPELVAALIAQEDDLDTQSQTETYVQTAEEMQ---TFDYSSMNQALYSFLDRLTE-

General information: TITO was launched using: RESULT: Template: 3L7N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1294 -26193 -20.24 -115.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -20.24 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_3L7N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L7N-query.scw PDB file : Tito_Scwrl_3L7N.pdb: