Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRPGKKSSSNKSRVMKNKYAHRSWLGAVCLLGCSLSYAAEEQFNDALNAANSGNTALLDQYQLAMQNDVLGYYPEYWKLNTNLGFQSPTSIVSFAQRYPQSAMAEKLAADYVEEKVKQADFASAQPILPYVSNPDQAENCALAQVRAKSGDAL-VFAEYKDVWLATESQPESCIGLGRMMLSSPLMSTQDKQQRLWVQLRAG-------LSGQALATAQTLGLNLSLAQLNQIQANPLNYLWSAPKTNDVDYAYLI--FALGRLANNDLGNAFANVQRVAQG---TPESVQKYLYRTVAYIGGTTVMKNNFNREVLQYFDASYGYPLSPEEAEIYARQAIRFSAWESLIRAIDSMSVSQKQEDRWQYWLARATEQRGDSNSKNTAQCIYKKLAESGDDYHNLLAKDRLGVRYNHQPYNDEPTASDLRRLDQNIHFNRAFTLRRINVNPTYTNREWNWAVRQAYLQHDDGLLLAAAKRAHDMGWYDRAIYAADRTTNKHNDTYRYVTPHKTNVVSHSYNAG--IDPAWAYGLMRQESRFVTSARSHVGAGGLMQIMPDTAKLIARQMGET--YNPAALSEMNTNIRYGTFYLSMIQGQLSNNPVLATAGYNAGPNRARRWQPDYQP-IEADQYTETIPLLETRDYVKHVMTNATHYGVILGQGAQSLIQRMKVIPTRSSP 1SLY Chain:A ((24-603)) --------------------------------------------------------VVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQSRFVNELARREDWRGLLAFSPEKPGTTEAQ-CNYYYAKWNTGQSEEAWQGAKELWLTGKSQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIA-----SAIISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNEDQIQE-LRDIVAW----RLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTWLARLPMEAKEKDEWRYWQADLLLERG--REAEAKEILHQLMQQRG--FYPMVAAQRIGEEYELK-IDKAPQNVD-SALTQGPEMARVRELMYWNLDNT-ARSEWANLVKSKSKTEQAQL----ARYAFNNQWWDLSVQAT--IAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAWNPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQQFGNNRIFSSAAYNAGLGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAYYRYFMG-------------------

General information: TITO was launched using: RESULT: Template: 1SLY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2504 42654 17.03 75.90 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 17.03 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_1SLY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SLY-query.scw PDB file : Tito_Scwrl_1SLY.pdb: