Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQVKIYANERTIIQYRELLSHAIHQALIEELKYPVEKRFQRFISLKPENFIYPSDRSQHYIIIELSMFAGRSPATKKQLIQTLFRNIEEQCKIAPQDIEITIFETPKENWGIRGKNADELLLNYQVNI 4LKB Chain:F ((25-142)) --QIKVYGLAEKLNPIKAELSNILHTSLIEVLQISPEKRFHRFFPLDKLDFYYPSDRTDNYLIIEIIMFEGRSVETKKQLLRDIFKKVDEKFGISVYDIEITLFEIPKQNWGIRGIPGDE---------

General information: TITO was launched using: RESULT: Template: 4LKB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 447 -65510 -146.55 -555.17 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain F : 0.82 3D Compatibility (PKB) : -146.55 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.655

(partial model without unconserved sides chains): PDB file : Tito_4LKB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LKB-query.scw PDB file : Tito_Scwrl_4LKB.pdb: