Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHFQFQTVPNIISGLGSIQELQNVLASKTYRKLLLVTDAGMIKNQLHLPILQILDTLKIEHVIYADVQADPPEQVVLDAANYAKQQNVDVIVGFGGGSSLDVAKVIALLAHPNQTQNLQEMYGVNQVKTARLPLILIPTTAGTGSEVTPISIVTTGETT-KMGIVSPVLYADTAILDATFTQNLPAHITAATGIDAMVHAIEAYTSKHKKNIYTDILAKHALKLLNHNLPKVLKNGNDLEARQNMLFGSMLAGQAFANSPVAAVHALAYPLGGHFHLSHGHTNALVLVEVLKFNAPYAKQYYAELMQWLDPYSNGST-DGLCDLFIDHMQNHLDRSGLTLKLSDLGIEESSLPRLAQDAMLQTRLLQNNPRDMTETAALAIYQAIY 3BFJ Chain:J ((4-387)) -RMFDYLVPNVNFFGPNAISVVGERCQLLGGKKALLVTDKGLRKDGAVDKTLHYLREAGIEVAIFDGVEPNPKDTNVRDGLAVFRREQCDIIVTVGGGSPHDCGKGIGIAATH--EGDLYQYAGIETLTNPLPPIVAVNTTAGTASEVTRHCVLTNTETKVKFVIVSWRNLPSVSINDPLLMIGKPAALTAATGMDALTHAVEAYISKD-ANPVTDAAAMQAIRLIARNLRQAVALGSNLQAREYMAYASLLAGMAFNNANLGYVHAMAHQLGGLYDMPHGVANAVLLPHVARYNLIANPEKFADIAELMGENITGLSTLDAAEKAIAAITRLSMDIGIPQHLRDLGVKETDFPYMAEMALKDG-NAFSNPRKGNEQEIAAIFRQAF

General information: TITO was launched using: RESULT: Template: 3BFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 2395 -146628 -61.22 -385.86 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain J : 0.75 3D Compatibility (PKB) : -61.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_3BFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BFJ-query.scw PDB file : Tito_Scwrl_3BFJ.pdb: