Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKYQIKLSQLIKGCFIYAIANYALAQPIVVEGVVPNEASKQAILMKMQSVYGADQVVDKIQVRPVAAPNGWSDSVTRVITPDLKKVSQGKLSVNGTRFELTGKMLNPTEIQPTIQSFQSLVQPPYQLFSQLSVNQAEQKIIDDALKNRIIEFESGSAVLTEAGQKILDEMAVALNKVGGKKVKIVGHTDSSGDATKNLKLSQDRALAVKNYLISKNIPADHLSTEGLGSSKPVADNTSAEGRKKNRRIEFTVL 4PWT Chain:B ((24-131)) --------------------------------------------------------------------------------------------------------------------------------------------------QKNNIVYFGFDKYDIGSDFAQMLDAHAAFLRSNPSDKVVVEGHADERGTPEYNIALGERRASAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDEAAFAKNRRAVLVY-

General information: TITO was launched using: RESULT: Template: 4PWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 473 -28946 -61.20 -268.01 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : -61.20 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_4PWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PWT-query.scw PDB file : Tito_Scwrl_4PWT.pdb: