Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGIKTIGVVGVGVIGASWTALFLYKGFKVKVYGPYPIDEELFKKRIQANLSDLLALDQQTDSSHHLQDIFLNLELYNNLKDAVIDADFIQENAPERLDLKQNLYQEITSYCPEKTLIASSSSGLKVSDFQKDATHPERIFLGHPFNPPHLLPLVEIVGGKLTDPQILKKASEFYQSLGKHPIVLNKEVKGHVANRLQAALWREAFSLVKEGVCSAEDVDIAITSGPGLRWALFGPYINMELANQKGFKEAIHHLGPPMTEWWNDMQN-FQHSEETTELLEEQTKELLTHY--KDIDLSQKRDKGLVDILKLKQQLELD 3F3S Chain:A ((2-312)) --MAGCVVIVGSGVIGRSWAMLFASGGFQVKLYDIEQQQIRNALENIRKEMKLLEQAGSLKGS-LSVEEQLSLISGCPNIQEAVEGAMHIQECVPEDLELKKKIFAQLDSIIDDRVILSSSTSCLMPSKLFAGLVHVKQCIVAHPVNPPYYIPLVELVPHPETAPTTVDRTHALMKKIGQCPMRVQKEVAGFVLNRLQYAIISEAWRLVEEGIVSPSDLDLVMSEGLGMRYAFIGPLETMHLNA-EGMLSYCDRYSEGIKHVLQTFGPIPEFSRATAEKVNQDMCMKVPDDPEHLAARRQWRDECLMRLAKLKSQ----

General information: TITO was launched using: RESULT: Template: 3F3S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1280 -128407 -100.32 -416.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -100.32 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_3F3S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F3S-query.scw PDB file : Tito_Scwrl_3F3S.pdb: