Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKLNQVNVMVKHEQSLAPAQPVSKLVRSMWARWVFIG----LAWLCIALGILGIFIPGLPTVDFIILAVFFAARGSEKLHQWFRN----------HRYVGPLIREWQEHRRIPKKAKYISTLSMSLAAGLMIWTIPHPWFV-YPAILCMVGVLAWMWLKK- 1AA7 Chain:A ((1-158)) MSLLTEVETYVL---SIIPSGPLKAEIAQRLED-VFAGKNTDLEVLMEWLKTRPILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDKAVKLYRKLKREITFHGAKEISLSYSAGALASCMGLIYNRMGAVTTEVAFGLVCATCEQIADSQ

General information: TITO was launched using: RESULT: Template: 1AA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 533 -57193 -107.30 -402.77 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -107.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_1AA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AA7-query.scw PDB file : Tito_Scwrl_1AA7.pdb: