Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQQQVKNEFPTLEDIHAAAERLDGLVVKTPFVFSETISKTLGAEMWLKFENLQFTASFKERGALNKLLSLSEQEKQHGVIAASAGNHAQGVAYHAQRTGVTATIVMPKSTPNVKVQRVREYGARVILHGQDFSEAAAEMHRVAQEESLTIIHPFDDAEIIAGQGTIALEMLEAVPDLDILVVPIGGGGLISGIAIAAKSINPKIKIIGVQSVVYPSMAKLLC-NYQLAVSMGSTVAEGIAVKTPGELTTQIAKHFVDDIVVVTEDMIEEAIALLLNIEKTVCEGAGATGIAAIMSRSDL--FLGHKVGVVLSGGNIDTRVMVSVLQRHLTRTGRMVRICVELSDNPGALARLTAIIAEQGGNIYELRHERFAATSRAKESAVSVDIELKSAPDLEPLIQAMQLEGYIVRKEEI 2GN0 Chain:B ((19-338)) -------DLPVAIEDILEAKKRLAGKIYKTGMPRSNYFSERCKGEIFLKFENMQRTGSFKIRGAFNKLSSLTEAEKRKGVVACSAGNHAQGVSLSCAMLGIDGKVVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVETEGRIFIPPYDDPKVIAGQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAIAIKSINPTIKVIGVQAENVHGMAASYYTGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVITEGAGALACAALLSGKLDSHIQNRKTVSIISGGNIDLSRVSQITG---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2143 -130855 -61.06 -412.79 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -61.06 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_2GN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GN0-query.scw PDB file : Tito_Scwrl_2GN0.pdb: