Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTERSPLRVGIGGPVGSGKTALTLNLCRALRDKYNMAVVTNDIYTKEDSNFLTRNEAMSPDRIVGVETGGCPHTAIREDASINLAAIDDLCEKFDGLELIIIESGGDNLAATFSPELSDLTLYVIDVAGGEKIPRKGGPGITKSDLLIINKTDLAPMVGANLDVMDQDAKRMRGEKPFLFSNMKTQDGLEEIIQFIEKQGLFKA 4HI0 Chain:F ((2-196)) ------VKIGVCGPVGSGKTALIEALTRHMSKDYDMAVITNDIYTKEDAEFMCKNSVMPRERIIGVETGGCPHTAIREDASMNLEAVEEMHGRFPNLELLLIESGGDNLSATFNPELADFTIFVIDVAEGDKIPRKGGPGITRSDLLVINKIDLAPYVGADLKVMERDSKKMRGEKPFIFTNIRAKEGLDDVIAWIKRNAL---

General information: TITO was launched using: RESULT: Template: 4HI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1037 -109305 -105.40 -560.54 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain F : 0.94 3D Compatibility (PKB) : -105.40 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_4HI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HI0-query.scw PDB file : Tito_Scwrl_4HI0.pdb: