Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPV-SGAKVGGKIELDFASSDND---KSENVRIRHAYLTYN-----NWLFGQTTSNFLS-SHAPEMID-FSTNI-----GGGTTRIPQVRYNYK-LA---PATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSGA-------GDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEG-KSYKDNRVGLMAKYSF 2ZFG Chain:A ((5-340)) ----------------------------------------------------------------------------------------------NKDGNKVDLYGKA-VGLHYFSKG-GEN-----------SYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNKTRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYLGKNERD--TARRSNGDGVGGSISYEY--EGFGIVGA--YGAADRTNLQEAQPLGNGKKAEQWATGLKYDAN-NIYLAANYGETRNATPITNKFT------NTSGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGI-----GDVDLVNYFEVGATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF

General information: TITO was launched using: RESULT: Template: 2ZFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1663 51287 30.84 167.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 30.84 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.134

(partial model without unconserved sides chains): PDB file : Tito_2ZFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZFG-query.scw PDB file : Tito_Scwrl_2ZFG.pdb: