Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNKIRGAIYGLLIGDAVGVPYEFHSALQLPLYSHIDMIPPKDFRRTYPDIPIGTWSDDGAQALCLLASLLHCQKLDSIDFANRLRNWYRYGYMAVDYQVFDIGVQTAQALQRYEAGVSVEEMANTDEHSNGNGALMRTLPLALWYRGSDQQLVELAYQQSHLTHAHLRSKICCALYCLWAKCLLKGQSVNNAWKSSISILMQLYKNSVNEQQELNHIIDSTH-EKKISGSGYVVDCLHSARFALQQD-NYEDVIKTAIALGQDTDTTACVAGGLAGIVFGYEGIPQIWLQQLRGKEILKPLLTQLEEA 2YZW Chain:A ((8-293)) -QDRRLGAFLGLAVGDALGAQVEGLPKGTFPEVREMKGGGPHR-------LPPGFWTDDTSQALCLAESLLQ-RGFDPKDQMDRYLRWYREGY------------ATRRALERYAA---TGDPYAGDEAGAGNGPLMRLAPLVLAYE-NHPDLLSLARRAARTTHGA-REALEATEVLAW---LLR-EALRGAPKEALLALEPF--RGADLHPALRRVVEGGFWEAPEEGPGYAPGTLAAALWAFARGRDFEEGMRLAVNLGGDADTVGAVYGQLAGAYYGLGAIPGRWLRALHLREEMEALALAL---

General information: TITO was launched using: RESULT: Template: 2YZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1662 -93866 -56.48 -345.10 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -56.48 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_2YZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YZW-query.scw PDB file : Tito_Scwrl_2YZW.pdb: