Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTLSMNYLQDARQHVLQQIRTACELAQREPETVQLLAVSKTHPSERLREMYAAGQRAFGENYLQEALDKIDA-L---QDLDIEWHFIGHVQRNKTKHLAEKFDWVHGVDRLIIAERLSNQRGDDQAALNICLQVNIDGQESKDGCAPEDVAELVAQMSQLPKIKLRGLMVIPAPDNT--GAFADAKKLFDAVKVQH-AHPEDWDTLSMGMSSDLEAAIAAGSTMVRVGTALFGARDYSQKG 1W8G Chain:A ((4-228)) --------IAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIAEAIDAGQRQFGENYVQEGVDKIRHFQELGVT-GLEWHFIGPLQSNKSRLVAEHFDWCHTIDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRLRGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYP-HIDTLSLGMSDDMEAAIAAGSTMVRIGTAIFGA-------

General information: TITO was launched using: RESULT: Template: 1W8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1088 -3553 -3.27 -16.30 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -3.27 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_1W8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W8G-query.scw PDB file : Tito_Scwrl_1W8G.pdb: