Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADIRITGRMVNFSRITFDTNDHDVIRQQLSNILNEG--SY-QGTVVIIDST----VEQELIALIQLLVSMGLQPMAVIDGILGDEARAIQFPVLPADQPLQRIKPTAEQVAIVEKPTSAQASVETKKPLNNNAVAHITSYHDEILRTGQSLVQDQGDIILKAGMNSGSEVIASGNIHIYGTVRGRVIAGAGGHAAARIFCQSLEAELVSIAGTYCVADDIPKHVVKKPVHIYLNEKQELEFEALEL 1HF2 Chain:B ((2-202)) ---VDFKMTKEGLVLLIKDYQNLEEVLNAISARITQMGGFFAKGDRISLMIENHNKHSQDIPRIVSHLRNLGLEVSQILVGSTVEGK-ENDL-----------------------------------KVQSRTTVESTGKVIKRNIRSGQTVVH-SGDVIVFGNVNKGAEILAGGSVVVFGKAQGNIRAGLNEGGQAVVAALDLQTSLIQIAGFITHSKGE----ENVPSIAHVKGNR-IVIEPFD-

General information: TITO was launched using: RESULT: Template: 1HF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1022 -34814 -34.06 -179.45 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -34.06 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1HF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HF2-query.scw PDB file : Tito_Scwrl_1HF2.pdb: