Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLWLDIGNTRLKYWITENQQIIEHAAELHLQSPADLLLGLIQHFKHQGLHRIGISSVLDTENNQRIQQIL-KWLEIPVVFAKVHAEYAGLQCGYEVPSQLGIDRWLQVLAVAEE-KENYCIIGCGTALTIDLTK-GKQHLGGYILPNLYLQRDALIQNTKGIKIPDSAF----DNLNPGNNTVDAVHHGILLGLISTIESIMQQS------PKKLLLTGGDAPLFAKFLQKYQPTVETDLLLKGLQQYIAHYPKD 2F9W Chain:B ((23-270)) SMILELDCGNSLIKWRVIEGAARSVA--GGLAESDDALVEQLTSQ-QALPVRACRLVSVRSEQETSQLVARLEQLFPVSALVASSGKQLAGVRNGYLDYQRLGLDRWLALVAAHHLAKKACLVIDLGTAVTSDLVAADGVHLGGYICPGMTLMRSQLRTHTRRIRYDDAEARRALASLQPGQATAEAVERGCLLMLRGFVREQYAMACELLGPDCEIFLTGGDAELVRDELAG-AR-IMPDLVFVGLALACPI----

General information: TITO was launched using: RESULT: Template: 2F9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1093 -72792 -66.60 -309.75 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -66.60 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_2F9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F9W-query.scw PDB file : Tito_Scwrl_2F9W.pdb: