Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFDHIAQPISY-QHIELSFPVHLDIKRLDLVHPQISGNKFFKLKYNLLAAKEQGLSSILTFGGAYSNHIAATAYAAHLFGLKSIGIIRG-EELAGKPLNPTLAKAQSLGMQLHFVSRHEYRLRDEANYL-KQLQQQFPQTYIIPEGGTNELA----VQGCQEILSQYDLEQYDVICCAVGTGGTISGL-IERSAAHQKV--LGFSALK-GDFLQQEIKQWTKKQNWSL---TDV------YCWGGYAKTSPELFTFIENFEEQYTVPLEPIYTGKMMFGLFDLIKNNYFPANTRILAIHSGGLQADIRNRSHA 1J0A Chain:A ((23-313)) -------PIQYLPNISREIGADVYIKRDDLTGLGIGGNKIRKLEYLLGDALSKGADVVITVGAVHSNHAFVTGLAAKKLGLDAILVLRGKEELKG---NYLLDKIMGIETRV-YDAKDSFELMKYAEEIAEELKREGRKPYVIPPGGASPIGTLGYVRAVGEIATQSEV-KFDSIVVAAGSGGTLAGLSLGLSILNEDIRPVGIAVGRFGEVMTSKLDNLIKEAAELLGVKVEVRPELYDYSFGEYGKITGEVAQIIRKVGTREGIILDPVYTGKAFYGLVDLARKGEL--GEKILFIHTGGISG--------

General information: TITO was launched using: RESULT: Template: 1J0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1495 -30624 -20.48 -113.00 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -20.48 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_1J0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J0A-query.scw PDB file : Tito_Scwrl_1J0A.pdb: