Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVNFNHETQPEFELPIKNYQEFYRFYLTEHR-----NIMSRCLHAVGSSIGLYFFSKAIRKKQAKYALYGLVSGYACAWVGHFMFEKNKPASFKQPLYSFISDWRMLSD--VATGRLSLIDRKHDKIPS 1AMZ Chain:A ((218-325)) ----------TDPQF-------TELMRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLLWLSQLQKDLGADAS-DEKLRDYI--WNTLNSGRVVPGYGHAVLRKTD----

General information: TITO was launched using: RESULT: Template: 1AMZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 -9541 -48.19 -94.47 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -48.19 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_1AMZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AMZ-query.scw PDB file : Tito_Scwrl_1AMZ.pdb: