Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEFIAVILITILAVISP--GADFAIVTKNSYLYGRKIGVFTSLGISLGVLVHVTYTLVAVAFVMTYTPQILNIVKYIGALYLIYIGYKTFTQKPVL-E----T--T--ALTAIGTFQAIKYGFFTNA-LNPKTTLFVISTYTQIVSLTTPKSILLAYGFFMSFAHFVWFSLVAVLFSSMLLRQKMLAKQVQINRVIGSILCVLGVILLFTKFQ 2N4X Chain:A ((14-198)) ------AFALGILSVFSPAVLPVVPLIFAGS-RGRALDAFLIVAGLTISMLILGYTASLF-F----------GFFRVVAMLFLLIFALILLSDELDEKVSIFASRMTSGLSWKIQTLPSFFFGMLLAFLWLPAILPFAGIAISQTLLSE-N---P-LVMLSYGLGMAVTIAAVFKMGEK--FVK---ANFQLIRKVTGAIVLLYLAYFALTEV-

General information: TITO was launched using: RESULT: Template: 2N4X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 594 -82955 -139.65 -479.51 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -139.65 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.122

(partial model without unconserved sides chains): PDB file : Tito_2N4X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N4X-query.scw PDB file : Tito_Scwrl_2N4X.pdb: