Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMTFIVAVQLKDSIVVAVDNKYLTLKDKEKDHFE-EHFSSKLYAWHS-GIIT-GTGEHYVIDKAVRLFINSADA-------------DI-----EKLPLCLNISRQIREMEVGQHEQIQSSKLLYSQYSENGAKLFAIEPTEETGKYQRTEFKENDLIIWLFNPNIQSISGNLKTLYSNPRPKVSFDRIEDWLDYYISAIAEIYEKQSCIDSWMSGSFDIFFQTEGEYFYKHIQNNPTVHLE 5NYJ Chain:G ((2-205)) ---TYAVAFRLERGLVFAADTRTNAG-V-D--NIAQYKKLQLWRQPGERVFVLLSAGNLAATQAVVSLINEHLSQETDDEVTTLFTAPNMYRAARVVGDAVREARSID-------GF--NTNFIFGGQIKGERPRLFQIYPEGNFIEA---TD-DT--PFFQIGEH-KYGKPILDRVAR---SD---MR----LGEAAKLMLLSFDSTLRSNLSVGMPIDLVIYERDT---------------

General information: TITO was launched using: RESULT: Template: 5NYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 835 -8078 -9.67 -46.43 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain G : 0.64 3D Compatibility (PKB) : -9.67 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_5NYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5NYJ-query.scw PDB file : Tito_Scwrl_5NYJ.pdb: