Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDELYPVSINELRQ-LGVHEKTAYRDLKEGINRLYERSINLSIDD----------KSIKMRWVQEVQFLDSQSVIGIRFSKPILPFISNL---SREFTKYALSDIAGINSGYGIRIYELLVQYRQI----GKRE-ISVENLRTMLEL---------GKKYPLFADFKKRVIDTAIDQINECSPLNV-TYEQ-KKTGRKVTSIIFSFKEKTKSISHQNTDVPKEFYKLTDAQINMFGNQLSRLHELSHLAQQGESYDDLAITIKDMLRDPKQQKQFLPYLKNLGFKL 2NRA Chain:C ((11-268)) -KKTKIRHRNELNHTLAQLPLPAKRVMYMALALIDSKEPLERGRVFKIRAEDLAALAKITPSLAYRQLKEGGKLLGASKISLRGDDIIALAKELNSEELDLNIIEWIAYSPDEGYLSLKFTRTIEPYISSLIGKKNKFTTQLLTASLRLSSQYSSSLYQLIRKHYSNFKKKN-YFIISVDELKEELIAYTFDKDGNIEYKYPDFPIFKRDVLNKAIAEIKKKTEISFVGFTVHEKEGRKISKLKFEFVVDED---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2NRA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 936 -19364 -20.69 -88.02 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -20.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_2NRA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NRA-query.scw PDB file : Tito_Scwrl_2NRA.pdb: